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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GRIK1 / GLUR5 Antibody (aa878‑889) IHC‑plus™ LS‑B8298
Note: This antibody replaces LS-C54918
GLUR5 antibody LS-B8298 is an unconjugated goat polyclonal antibody to GLUR5 (GRIK1) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8298-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

GLUR5 antibody LS-B8298 is an unconjugated goat polyclonal antibody to GLUR5 (GRIK1) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About GRIK1 / GLUR5
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. P39086 NM_000830 NP_000821.1

GRIK1 Antibody, GluR-5 Antibody, GLUR5 Antibody, EEA3 Antibody, GluK1 Antibody, Glutamate receptor 5 Antibody, EAA3 Antibody, GLR5 Antibody


Specifications

Target
Human GRIK1 / GLUR5
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1:8000) & (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GRIK1 / GLUR5 antibody was raised against synthetic peptide QCKQTHPTNSTS from an internal region (near the C-terminus) of human GRIK1 (NP_000821.1; NP_783300.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Horse (100%); Turkey (92%); Opossum (83%).
Blocking Peptide
LS-E26447 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8298. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa878-889
Specificity
Human GRIK1. This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-GRIK1 / GLUR5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8298 dilution 3.75 ug/ml.
Anti-GRIK1 / GLUR5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8298 dilution 3.75 ug/ml.

Western blot

Antibody (1 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-GRIK1 / GLUR5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8298 dilution 3.75 ug/ml.
Anti-GRIK1 / GLUR5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8298 dilution 3.75 ug/ml.

Western blot

Antibody (1 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-GRIK1 / GLUR5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8298 dilution 3.75 ug/ml.
Anti-GRIK1 / GLUR5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8298 dilution 3.75 ug/ml.

Western blot

Antibody (1 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-GRIK1 / GLUR5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8298 dilution 3.75 ug/ml.
Anti-GRIK1 / GLUR5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8298 dilution 3.75 ug/ml.

Western blot

Antibody (1 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/20/2018

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