Target
Mouse GRB4 / NCK2
Synonyms
NCK2 | GRB4 | NCKbeta | NCK beta | Nck-2 | Cytoplasmic protein NCK2 | NCK adaptor protein 2
Reactivity
Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide corresponding to aa175-190 of murine Nck beta [EAPSFLSLRRGTALSN] (KLH-conjugated). Percent identity by BLAST analysis: Mouse, Rat (100%); Hamster (94%).
Specificity
Recognizes mouse Nck beta, Mr 47kD. Species sequence homology: human.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation (1:100)
Usage
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 1:100. Immunoprecipitates. Nck beta from 1-2 mg of mouse tissue homogenates.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee