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Anti-GRB4 / NCK2 Antibody (aa119-276) IHC-plus™ LS-B1286

Note: This antibody replaces LS-C33804

Ordering

Wt. Vol. Conc. Price
50 µg 250 µl 0.2 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GRB4 / NCK2 Antibodies

Anti-GRB4 / NCK2 Antibody (aa175-190) LS-C25734
Rabbit Polyclonal (IgG) to Mouse GRB4 / NCK2
Mouse, Rat
Western blot, Immunoprecipitation
Unconjugated
Anti-GRB4 / NCK2 Antibody (aa119-276) LS-C50320
Rabbit Polyclonal to Human GRB4 / NCK2
Human, Mouse, Rat
IHC
Unconjugated
Immunohistochemistry Image
Anti-GRB4 / NCK2 Antibody (aa63-92) LS-C164774
Rabbit Polyclonal to Human GRB4 / NCK2
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human GRB4 / NCK2
Human
IHC - Paraffin
Unconjugated

Details

Human GRB4 / NCK2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

IHC - Paraffin (5 µg/ml)

Specificity and Use

GRB4 / NCK2 antibody was raised against recombinant human NCK2.
aa119-276
Amino acids 119 to 276 of human NCK2
Immunohistochemistry: LS-B1286 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1286 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
+4°C, avoid freezing
For research use only.

About GRB4 / NCK2

O43639 NM_003581 NP_003572.2

NCK2 Antibody, GRB4 Antibody, NCKbeta Antibody, NCK beta Antibody, Nck-2 Antibody, Cytoplasmic protein NCK2 Antibody, NCK adaptor protein 2 Antibody

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

Immunohistochemistry

Immunohistochemistry
Anti-NCK2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1286 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/11/2016

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