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Anti-GRB2 Antibody IHC-plus™ LS-B3068

Note: This antibody replaces LS-C7285

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Most Popular GRB2 Antibodies

Anti-GRB2 Antibody LS-C7287
Mouse Monoclonal (IgG) to Human GRB2
Human
Immunoprecipitation
Unconjugated
Anti-GRB2 Antibody (aa206-217) IHC-plus™ LS-B3923
Goat Polyclonal to Human GRB2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GRB2 Antibody IHC-plus™ LS-B4079
Rabbit Polyclonal to Human GRB2
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1,k) to Rat GRB2
Rat, Human, Mouse, Bovine, Hamster
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Rat GRB2
Mouse
Rat, Human, Mouse, Bovine, Hamster (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

GRB2 antibody was raised against recombinant rat GRB2/Ash.
Recognizes the N-terminal SH3 region of rat Grb2 at 23-25kD.
Immunohistochemistry: LS-B3068 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3068 was determined to be 5 ug/ml. Western Blot: 0.5-2 mg/ml. Detects GRB2 (23-25kD) in RIPA lysates of non-stimulated human A431 carcinoma cells. Previous lots detected GRB2 in RIPA lysates of mouse 3T3 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells and bovine brain extract.

Packaging

PBS, 50% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GRB2

P62993 NM_002086 NP_002077.1

GRB2 Antibody, Abundant SRC homology Antibody, Adapter protein GRB2 Antibody, ASH Antibody, EGFRBP-GRB2 Antibody, MST084 Antibody, MSTP084 Antibody, Protein Ash Antibody, SH2/SH3 adapter GRB2 Antibody, Grb3-3 Antibody, HT027 Antibody, NCKAP2 Antibody

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

Immunohistochemistry

Immunohistochemistry
Anti-GRB2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3068 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-GRB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3068 concentration 5 ug/ml.

Requested From: 
Date Requested: 3/28/2017

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