Monoclonal Rat anti‑Mouse Gr‑1 Antigen Antibody (clone RB6‑8C5, IHC, IF, WB) LS‑C44779
Monoclonal Rat anti‑Mouse Gr‑1 Antigen Antibody (clone RB6‑8C5, IHC, IF, WB) LS‑C44779
Note: This antibody replaces LS-C57929, LS-C44776, LS-C123654
Antibody:
Gr-1 Antigen Rat anti-Mouse Monoclonal (RB6-8C5) Antibody
Application:
IHC, IHC-Fr, IF, WB, IP, Flo
Format:
Unconjugated, Unmodified
Overview
Antibody:
Gr-1 Antigen Rat anti-Mouse Monoclonal (RB6-8C5) Antibody
Application:
IHC, IHC-Fr, IF, WB, IP, Flo
Format:
Unconjugated, Unmodified
Specifications
Description
Gr-1 Antigen antibody LS-C44779 is an unconjugated rat monoclonal antibody to mouse Gr-1 Antigen. Validated for Flow, IF, IHC, IP and WB.
Target
Mouse Gr-1 Antigen
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
RPE.
Purification
Protein G purified
Immunogen
Normal murine bone marrow cells
Specificity
Recognizes the mouse Gr-1 antigen, a 21-25kD cell surface protein that belongs to the Ly-6 family of proteins. Clone Reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been demonstrated. The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Expression of Gr-1 on a subpopulation of lymphocytes has also been reported. Clone RB6-8C5 has been reported to deplete mouse mature neutrophils in vivo. XXX recommend the use of MCA2387EL for functional studies.
Applications
- IHC
- IHC - Frozen
- Immunofluorescence
- Western blot
- Immunoprecipitation
- Flow Cytometry (1:100 - 1:200)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
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Species:
Mouse
Applications:
Flow Cytometry
Source:
E. coli
Tag:
His, N-Terminal
Reactivity:
Human
Range:
2.47-200 ng/ml
Reactivity:
Mouse
Range:
0.16-10 ng/ml
Source:
Escherichia coli
Tag:
None
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 9/19/2024