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Anti-GPX3 Antibody (Internal) IHC-plus™ LS-B3036

Note: This antibody replaces LS-C54936, LS-C66686
See our new GPX3 ELISA Kits
Rat GPX3 ELISA Kit - LS-F6289
Human GPX3 ELISA Kit - LS-F7125
Human GPX3 EIA Kit - LS-F11571
Mouse GPX3 ELISA Kit - LS-F11572
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

GPX3 Antibody LS-B3036 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-GPX3 antibody IHC of human placenta.
Immunohistochemistry
Anti-GPX3 antibody IHC of human colon.
Western blot
Antibody (1 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer).

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Goat Polyclonal to Human GPX3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human GPX3
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig (tested or 100% immunogen sequence identity)
Rabbit, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (1 - 3 µg/ml), ELISA (1:1000)

Specificity and Use

GPX3 antibody was raised against synthetic peptide C-NQFGKQEPGENSE from an internal region of human GPX3 (NP_002075.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig (100%); Rabbit, Opossum, Chicken (92%); Hamster, Zebrafish, Pseudomonas (85%).
LS-E26292 - Lyophilized - 100 µg - $145.00
Internal
Human GPX3. This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6
Immunohistochemistry: LS-B3036 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3036 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About GPX3

P22352 NM_002084 NP_002075.2

GPX3 Antibody, Glutathione peroxidase 3 Antibody, GSHPx-3 Antibody, GPXP Antibody, GSHPx-P Antibody, GPx-3 Antibody, GPx-P Antibody, Plasma glutathione peroxidase Antibody

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme. GPX3 expression appears to be tissue-specific.

Immunohistochemistry

Immunohistochemistry
Anti-GPX3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3036 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-GPX3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3036 concentration 5 ug/ml.

Western blot

Western blot
Antibody (1 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 5/25/2015

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