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Anti-GPR44 / CRTH2 Antibody (N-Terminus) IHC-plus™ LS-B3209

Note: This antibody replaces LS-C19672

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GPR44 / CRTH2 Antibodies

Anti-GPR44 / CRTH2 Antibody (N-Terminus) IHC-plus™ LS-A3528
Rabbit Polyclonal to Human GPR44 / CRTH2
Human
IHC - Paraffin, ICC
Unconjugated
Immunohistochemistry Image
Anti-GPR44 / CRTH2 Antibody (Extracellular Domain) IHC-plus™ LS-A3529
Rabbit Polyclonal to Human GPR44 / CRTH2
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-GPR44 / CRTH2 Antibody (N-Terminus) IHC-plus™ LS-B3209
Rabbit Polyclonal to Human GPR44 / CRTH2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human GPR44 / CRTH2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human GPR44 / CRTH2
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

GPR44 / CRTH2 antibody was raised against synthetic peptide from human GPR44 / CRTH2.
LS-E30305 - Liquid - 50 µg 0.2 mg/ml - $145.00
N-Terminus
18 amino acid peptide from near the amino terminus of human CRTH2.
Immunohistochemistry: LS-B3209 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3209 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

Publications (1)

1
PGD2-CRTH2 pathway promotes tubulointerstitial fibrosis. Ito H, Yan X, Nagata N, Aritake K, Katsumata Y, Matsuhashi T, Nakamura M, Hirai H, Urade Y, Asano K, Kubo M, Utsunomiya Y, Hosoya T, Fukuda K, Sano M. Journal of the American Society of Nephrology : JASN. 2012 Nov;23:1797-809. [Full Text Article] [PubMed:22997255] [PMC:PMC3482731]

About GPR44 / CRTH2

Q9Y5Y4 NM_004778 NP_004769.2

PTGDR2 Antibody, CD294 Antibody, CRTH2 Antibody, CD294 antigen Antibody, DP2 Antibody, DL1R Antibody, G protein-coupled receptor 44 Antibody, G-protein coupled receptor 44 Antibody, HCRTH2 Antibody, DP2 prostaglandin receptor Antibody, GPR44 Antibody, Prostaglandin D2 receptor 2 Antibody

Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin-sensitive decreases in cAMP levels and Ca2+ mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, ADRBK1/GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses.

Immunohistochemistry

Immunohistochemistry
Anti-GPR44 / CRTH2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3209 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-GPR44 / CRTH2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3209 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-GPR44 / CRTH2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3209 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of CRTH2 in Human Heart cells with CRTH2 antibody at 20 ug/ml.

Western blot

Western blot
Western blot of CRTH2 in Jurkat cell lysate with LS-B3209 at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.
 

Requested From: United States
Date Requested: 12/3/2016

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