LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
GPR44 / CRTH2 Antibody (C‑Terminus) IHC‑plus™ LS‑A153
CRTH2 antibody LS-A153 is an unconjugated rabbit polyclonal antibody to human CRTH2 (GPR44). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A153-50
50 µg (1 mg/ml)
Unavailable
In Stock for Immediate Shipment

Popular GPR44 / CRTH2 Products

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, ICC
Anti-GPR44 / CRTH2 antibody LS-A3529 IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin
Anti-GPR44 / CRTH2 antibody LS-A3530 IHC of human nasal mucosa, eosinophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, ICC
Anti-GPR44 / CRTH2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3209 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

CRTH2 antibody LS-A153 is an unconjugated rabbit polyclonal antibody to human CRTH2 (GPR44). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
About GPR44 / CRTH2
Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin-sensitive decreases in cAMP levels and Ca2+ mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, ADRBK1/GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses. Q9Y5Y4 NM_004778 NP_004769.2

PTGDR2 Antibody, CD294 Antibody, CRTH2 Antibody, CD294 antigen Antibody, DP2 Antibody, DL1R Antibody, G protein-coupled receptor 44 Antibody, G-protein coupled receptor 44 Antibody, HCRTH2 Antibody, DP2 prostaglandin receptor Antibody, GPR44 Antibody, Prostaglandin D2 receptor 2 Antibody


Specifications

Target
Human GPR44 / CRTH2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (15 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GPR44 / CRTH2 antibody was raised against synthetic 20 amino acid peptide from C-terminal cytoplasmic domain of human GPR44 / CRTH2. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Pig (85%); Elephant (80%).
Blocking Peptide
LS-E28308 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A153. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human GPR44 / CRTH2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except KCNN2 (50%).
Usage
Immunohistochemistry: LS-A153 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A153 was determined to be 15 ug/ml.
Presentation
PBS, less than 0.1% sodium azide.
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images

Immunohistochemistry

Anti-GPR44 / CRTH2 antibody LS-A153 IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR44 / CRTH2 antibody LS-A153 IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR44 / CRTH2 antibody IHC of human tonsil mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR44 / CRTH2 antibody IHC of human tonsil mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR44 / CRTH2 antibody LS-A153 IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR44 / CRTH2 antibody LS-A153 IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR44 / CRTH2 antibody IHC of human tonsil mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR44 / CRTH2 antibody IHC of human tonsil mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR44 / CRTH2 antibody LS-A153 IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR44 / CRTH2 antibody LS-A153 IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR44 / CRTH2 antibody IHC of human tonsil mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR44 / CRTH2 antibody IHC of human tonsil mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR44 / CRTH2 antibody LS-A153 IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR44 / CRTH2 antibody LS-A153 IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR44 / CRTH2 antibody IHC of human tonsil mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR44 / CRTH2 antibody IHC of human tonsil mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: 
Date Requested: 7/19/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number