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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPI Antibody (C‑Terminus) LS‑C135916
GPI antibody LS-C135916 is an unconjugated rabbit polyclonal antibody to GPI from human, dog, guinea pig and other species. Validated for WB.
Catalog
Size
Price
LS-C135916-50
50 µg
Unavailable

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Product Description

GPI antibody LS-C135916 is an unconjugated rabbit polyclonal antibody to GPI from human, dog, guinea pig and other species. Validated for WB.
About GPI
GPI is a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. P06744 NM_000175 NP_000166.2

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Specifications

Target
Human GPI
Host
Rabbit
Reactivity
Human, Monkey, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
GPI antibody was raised against synthetic peptide from C-Terminus of human GPI (P06744, NP_000166). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Rabbit, Guinea pig (100%); Orangutan (92%); Bovine (91%); Panda, Dog, Pig (85%).
Blocking Peptide
LS-E22329 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135916. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human GPI
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

GPI antibody LS-C135916 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GPI antibody LS-C135916 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GPI antibody LS-C135916 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GPI antibody LS-C135916 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GPI antibody LS-C135916 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GPI antibody LS-C135916 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GPI antibody LS-C135916 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GPI antibody LS-C135916 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/17/2018

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