Rat GLUR2 + GLUR3

Rabbit

Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

Carboxy terminus peptide of rat GluR2 conjugated to BSA with glutaraldehyde (EGYNVYGIESVKI). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish (100%); Turkey, Stickleback, Trachemys (92%).

aa871-883

Recognizes both GluR2 and GluR3 which have nearly identical carboxy terminal sequences. Western blot analysis of rat brain shows two poorly separated bands which co-migrate with GluR2 and GluR3 expressed in transfected cells. GluR2 migrates slightly ahead of GluR3. No cross-reaction with GluR1 or GluR4. Species Reactivity: Human, rat, monkey, gerbil and hamster.

• IHC
• IHC - Frozen
• Western blot

Can be used for immunohistochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 ug/ml. Western blot analysis can be done at final concentrations of 0.1-1.0 ug/ml. Labels a band at approximately 108kD. The antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells.

PBS, pH 7.2, 0.1% Sodium Azide

Reconstitute in deionized water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.