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Anti-GLS / Glutaminase Antibody (aa516-545) LS-C166517

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GLS / Glutaminase Antibodies

Anti-GLS / Glutaminase Antibody (C-Terminus) LS-C111602
Rabbit Polyclonal to Human GLS / Glutaminase
Human, Monkey, Mouse, Rat, Dog, Guinea pig, Horse, Pig, Rabbit
Western blot
Unconjugated
Western blot Image
Anti-GLS / Glutaminase Antibody (aa516-545) LS-C166517
Rabbit Polyclonal to Human GLS / Glutaminase
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human GLS / Glutaminase
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human GLS / Glutaminase
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

GLS / Glutaminase antibody was raised against kLH-conjugated synthetic peptide between amino acids 516-545 from C-terminal region of human GLS.
LS-E9792 - Lyophilized - 100 µg - $145.00
aa516-545
Human GLS / Glutaminase 2

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About GLS / Glutaminase

O94925 NM_014905 NP_055720.3

GLS Antibody, AAD20 Antibody, GLS1 Antibody, GAM Antibody, Glutaminase C Antibody, GAC Antibody, K-glutaminase Antibody, KIAA0838 Antibody, L-glutamine amidohydrolase Antibody, Glutaminase Antibody, KGA Antibody

GLS / Glutaminase is the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

Immunohistochemistry

Immunohistochemistry
GLS Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunohistochemistry

Immunohistochemistry
Immunohistochemical of paraffin-embedded H.kidney section using GLS Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of GLS Antibody with HeLa cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence

Immunofluorescence
Fluorescent image of HeLa cells stained with XAF1 GLS Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunofluorescence

Immunofluorescence
Fluorescent image of HeLa cells stained with XAF1 GLS Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot

Western blot
Western blot of lysates from 293, 293T, HeLa cell line , human brain and mouse brain tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

Western blot
Western blot of GLS Antibody in mouse liver tissue lysates (35 ug/lane). GLS (arrow) was detected using the purified antibody.

Western blot

Western blot
Western blot of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

Western blot
Western blot of lysates from human brain and mouse brain tissue lysate (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

Western blot
Western blot of lysate from HeLa cell line, using GLS Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot

Western blot
Western blot of lysates from 293T, HeLa cell line (from left to right), using GLS Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Flow Cytometry

Flow Cytometry
GLS Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/11/2016

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