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Anti-GLI3 Antibody LS-C36438


Wt. Vol. Conc. Price
100 µg 100 µl - $695
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular GLI3 Antibodies

Anti-GLI3 Antibody (aa41-57) IHC-plus™ LS-B73
Rabbit Polyclonal to Human GLI3
Human, Dog, Chicken, Xenopus, Quail, Squirrel monkey
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image
Anti-GLI3 Antibody (aa1-479) LS-C150112
Goat Polyclonal (IgG) to Human GLI3
Human, Mouse
ICC, Western blot, Immunoprecipitation
Immunocytochemistry Image
Anti-GLI3 Antibody (aa975-1025) LS-C290096
Rabbit Polyclonal (IgG) to Human GLI3
Western blot, Immunoprecipitation

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human GLI3
Human, Mouse
ICC, Western blot, ELISA


Human GLI3
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Affinity purified


  • ICC (10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

GLI3 antibody was raised against e. coli-derived rhGLI-3.
Recognizes human and mouse GLI-3 in the applications listed. In direct ELISAs and Western Blots, the antibody shows less than 1% cross-reactivity with rhGLI-1 and rhGLI-2.
Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Western Blot: 0.1-0.2 ug/ml. Used in conjunction with the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 5 ng/lane under non- reducing and reducing conditions. Immunocytochemistry: 10 ug/ml detects human GLI-3 in HeLa cells. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: 0.5-1.0 ug/ml. Used in conjunction the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 1 ng/well.


100 µl deionized water
For research use only.

About GLI3

P10071 NM_000168 NP_000159.3

GLI3 Antibody, ACLS Antibody, GCPS Antibody, GLI-Kruppel family member GLI3 Antibody, GLI3 full length protein Antibody, GLI3-190 Antibody, GLI family zinc finger 3 Antibody, Gli-3 Antibody, GLI3FL Antibody, PAP-A Antibody, PAPA1 Antibody, PHS Antibody, PPDIV Antibody, Transcriptional activator GLI3 Antibody, PAPA Antibody, PAPB Antibody, GLI3 form of 190 kDa Antibody, Oncogene GLI3 Antibody, Zinc finger protein GLI3 Antibody

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor.

Requested From: United States
Date Requested: 10/23/2016

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