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Anti-GLI3 Antibody (aa41-57) IHC-plus™ LS-B73

Note: This antibody replaces LS-C19016, LS-C66534, LS-C56904

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

GLI3 Antibody LS-B73 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-GLI3 antibody IHC of human brain-glioblastoma.
Immunofluorescence
Anti-Gli-3 Antibody - Immunofluorescence.
Immunofluorescence
Anti-Gli-3 Antibody - Immunofluorescence.
Western blot
Anti-Gli-3 Antibody - Western Blot.

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Rabbit Polyclonal to Human GLI3
Human, Dog, Chicken, Xenopus, Quail, Squirrel monkey
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human GLI3
Rabbit
Human, Dog, Chicken, Xenopus, Quail, Squirrel monkey (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1.25 µg/ml), ICC, Immunofluorescence, Western blot (1:500 - 1:2000), ELISA (1:6000 - 1:30000)

Specificity and Use

GLI3 antibody was raised against synthetic peptide from human GLI3.
aa41-57
Amino acids 41-57 of human Gli-3 protein.
Immunohistochemistry: LS-B73 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B73 was determined to be 1.25 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GLI3

P10071 NM_000168 NP_000159.3

GLI3 Antibody, ACLS Antibody, GCPS Antibody, GLI-Kruppel family member GLI3 Antibody, GLI3 full length protein Antibody, GLI3-190 Antibody, GLI family zinc finger 3 Antibody, Gli-3 Antibody, GLI3FL Antibody, PAP-A Antibody, PAPA1 Antibody, PHS Antibody, PPDIV Antibody, Transcriptional activator GLI3 Antibody, PAPA Antibody, PAPB Antibody, GLI3 form of 190 kDa Antibody, Oncogene GLI3 Antibody, Zinc finger protein GLI3 Antibody

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor.

Immunohistochemistry

Immunohistochemistry
Anti-GLI3 antibody IHC of human brain-glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B73 concentration 1.25 ug/ml.

Immunofluorescence

Immunofluorescence
Anti-Gli-3 Antibody - Immunofluorescence. Anti-Gli-3 Antibody Immunofluorescence image showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - in green and staining of Anti-Gli-3 (RABBIT) Antibody - LS-B73 in red.

Immunofluorescence

Immunofluorescence
Anti-Gli-3 Antibody - Immunofluorescence. Anti-Gli-3 Antibody Immunofluorescence image one showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - in green. Immunofluorescence image two showing MCF-7 cell staining of Anti-Gli-3 (RABBIT) Antibody - LS-B73 in red. Immunofluorescence image three showing MCF-7 cell superimposed staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - in green and staining of Anti-Gli-3 (RABBIT) Antibody - LS-B73 in red.

Western blot

Western blot
Anti-Gli-3 Antibody - Western Blot. Western blot of anti-Gli-3 antibody shows detection of multiple bands in human lung lysate believed to be Gli-3. Lanes contain 20 ug of whole cell lysates from 1 - human brain, 2 - human lung, 3 - human spleen, 4 - mouse brain and 5 - mouse lung. While no recognizable staining can be seen on mouse tissue, human lung shows what may be truncated Gli-3 (~80kD). This identity of the strong band at ~50 kD is unknown. After blocking, the membrane was probed with the primary antibody diluted to 1:500. For detection use HRP Gt-a-Rabbit IgG (LS-C60865). Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Requested From: United States
Date Requested: 8/29/2015

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