LSBio The Immunohistochemistry Antibody Company
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-GLI3 Antibody (aa41-57) IHC-plus™ LS-B73

Note: This antibody replaces LS-C19016, LS-C66534, LS-C56904


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human GLI3
Human, Dog, Chicken, Xenopus, Quail, Squirrel monkey
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA


Human GLI3
Human, Dog, Chicken, Xenopus, Quail, Squirrel monkey (tested or 100% immunogen sequence identity)
Immunoaffinity purified


IHC - Paraffin (1.25 µg/ml), ICC, Immunofluorescence, Western blot (1:500 - 1:2000), ELISA (1:6000 - 1:30000)

Specificity and Use

GLI3 antibody was raised against synthetic peptide from human GLI3.
Amino acids 41-57 of human Gli-3 protein.
Immunohistochemistry: LS-B73 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B73 was determined to be 1.25 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GLI3

P10071 NM_000168 NP_000159.3

GLI3 Antibody, ACLS Antibody, GCPS Antibody, GLI-Kruppel family member GLI3 Antibody, GLI3 full length protein Antibody, GLI3-190 Antibody, GLI family zinc finger 3 Antibody, Gli-3 Antibody, GLI3FL Antibody, PAP-A Antibody, PAPA1 Antibody, PHS Antibody, PPDIV Antibody, Transcriptional activator GLI3 Antibody, PAPA Antibody, PAPB Antibody, GLI3 form of 190 kDa Antibody, Oncogene GLI3 Antibody, Zinc finger protein GLI3 Antibody

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor.


Anti-GLI3 antibody IHC of human brain-glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B73 concentration 1.25 ug/ml.


Anti-Gli-3 Antibody - Immunofluorescence. Anti-Gli-3 Antibody Immunofluorescence image showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - in green and staining of Anti-Gli-3 (RABBIT) Antibody - LS-B73 in red.


Anti-Gli-3 Antibody - Immunofluorescence. Anti-Gli-3 Antibody Immunofluorescence image one showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - in green. Immunofluorescence image two showing MCF-7 cell staining of Anti-Gli-3 (RABBIT) Antibody - LS-B73 in red. Immunofluorescence image three showing MCF-7 cell superimposed staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - in green and staining of Anti-Gli-3 (RABBIT) Antibody - LS-B73 in red.

Western blot

Western blot
Anti-Gli-3 Antibody - Western Blot. Western blot of anti-Gli-3 antibody shows detection of multiple bands in human lung lysate believed to be Gli-3. Lanes contain 20 ug of whole cell lysates from 1 - human brain, 2 - human lung, 3 - human spleen, 4 - mouse brain and 5 - mouse lung. While no recognizable staining can be seen on mouse tissue, human lung shows what may be truncated Gli-3 (~80kD). This identity of the strong band at ~50 kD is unknown. After blocking, the membrane was probed with the primary antibody diluted to 1:500. For detection use HRP Gt-a-Rabbit IgG (LS-C60865). Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Requested From: United States
Date Requested: 7/27/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number