LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-GLI / GLI1 Antibody (aa1-234) LS-C149959

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GLI / GLI1 Antibodies

Anti-GLI / GLI1 Antibody LS-C180157
Rabbit Polyclonal to Human GLI / GLI1
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GLI / GLI1 Antibody (aa755-1106, clone 1C1) LS-C339074
Mouse Monoclonal [clone 1C1] (IgG1) to Human GLI / GLI1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GLI / GLI1 Antibody (aa755-1106, clone 4E2) IHC-plus™ LS-B13058
Mouse Monoclonal [clone 4E2] (IgG1) to Human GLI / GLI1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human GLI / GLI1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human GLI / GLI1
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Immunoprecipitation

Specificity and Use

GLI / GLI1 antibody was raised against e. coli-derived recombinant human GLI1 Met1-Glu234 Accession # P08151.
aa1-234
Detects human GLI1 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human GLI3.
Western Blot, 0.1 µg/ml, Recombinant Human GLI-1. Chromatin Immunoprecipitation (ChIP), 5 µg/106 cells. Immunohistochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About GLI / GLI1

P08151 NM_005269 NP_005260.1

GLI1 Antibody, Gli-1 Antibody, GLI family zinc finger 1 Antibody, Glioma-associated oncogene Antibody, Glioma-associated oncogene 1 Antibody, Oncogene GLI Antibody, GLI Antibody, Zinc finger protein GLI1 Antibody

Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.

Immunohistochemistry

Immunohistochemistry
GLI-1 in Human Skin. GLI-1 was detected in immersion fixed paraffin-embedded sections of human skin using Human GLI-1 Antigen Affinity-purified Polyclonal Antibody at 5 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Detection of GLI-1-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin for 30 minutes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-1/DNA complexes were immunoprecipitated using 5 ug Goat Anti-Human GLI-1 Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The Bcl-2 promoter was detected by standard PCR.

Requested From: United States
Date Requested: 12/10/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number