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Anti-GK / Glycerol Kinase Antibody (aa461-510) IHC-plus™ LS-B9143

Note: This antibody replaces LS-C118858
Catalog Size Price
LS-B9143-50 50 µl Unavailable

Most Popular GK / Glycerol Kinase Antibodies

Anti-GK / Glycerol Kinase Antibody (aa461-510) IHC-plus™ LS-B9143
Rabbit Polyclonal (IgG) to Human GK / Glycerol Kinase
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Anti-GK / Glycerol Kinase Antibody (C-Terminus) LS-C352244
Rabbit Polyclonal to Human GK / Glycerol Kinase
Human, Mouse, Rat, Bovine, Dog, Pig
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescent analysis of Glycerol Kinase 1 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Anti-GK / Glycerol Kinase Antibody LS-C416334
Rabbit Polyclonal to Human GK / Glycerol Kinase
Human, Mouse, Rat
ICC, Immunofluorescence, Western blot
Unconjugated
Western blot of 293 cell lysate using GK Antibody
Anti-GK / Glycerol Kinase Antibody LS-C662594
Rabbit Polyclonal to Human GK / Glycerol Kinase
Human
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry - Anti-Glycerol kinase Picoband antibody
Anti-GK / Glycerol Kinase Antibody LS-C678082
Rabbit Polyclonal (IgG) to Human GK / Glycerol Kinase
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry of paraffin-embedded human thyroid tissue at dilution of 1:100

100% Guaranteed
Rabbit Polyclonal (IgG) to Human GK / Glycerol Kinase
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated

Details

Human GK / Glycerol Kinase
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)

Specificity and Use

Synthetic peptide from human GK (aa461-510).
aa461-510
GK Antibody detects endogenous levels of total GK protein.

Packaging

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Store at -20°C for up to 1 year.
For research use only.

About GK / Glycerol Kinase

P32189 NM_000167 NP_000158.1

GK Antibody, GKD Antibody, GK1 Antibody, Glycerokinase Antibody, Glycerol kinase Antibody

GK / Glycerol Kinase belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunohistochemistry

Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunofluorescence

Immunofluorescence of HeLa cells, using GK Antibody. The picture on the right is treated with the synthesized peptide.
Immunofluorescence of HeLa cells, using GK Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from 293/Jurkat cells, using GK Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from 293/Jurkat cells, using GK Antibody. The lane on the right is treated with the synthesized peptide.

Requested From: 
Date Requested: 5/25/2018

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