(tested or 100% immunogen sequence identity)
- Immunofluorescence (0.5 - 2 µg/ml)
- Western blot (0.5 - 2 µg/ml)
GJC3 / GJE1 antibody was raised against synthetic peptide derived from the C-terminal sequence of mouse Cx29.
Reacts with mouse Cx29. Peptide inhibition studies were used to confirm the antibody's specificity. Reactivity is confirmed with mouse brain or sciatic nerve homogenates. In Western blotting, two bands are usually observed. A band at ~29 kD is the connexin29 monomer, while a second band at ~58 kD corresponds to the Cx29 dimer.
Suitable for use in Immunofluorescence, ELISA and Western Blot. Immunofluorescence: 0.5-2 ug/ml. Western Blot: 0.5-2 ug/ml. Positive control: Mouse brain or sciatic nerve homogenates.
PBS, pH 7.4, 0.09% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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