Target
Mouse GJC2
Synonyms
GJC2 | Cx47 | Connexin-46.6 | Connexin-47 | CX46.6 | Gap junction protein alpha 12 | GJA12 | Gap junction gamma-2 protein | LMPH1C | Gap junction alpha-12 protein | HLD2 | PMLDAR | SPG44
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
Synthetic peptide derived from the C-Terminus of the mouse Connexin 47 protein.
Specificity
Reacts with the mouse Connexin 47 protein. On Western blots of mouse thalamus homogenates and Connexin 47-transfected HeLa cells, it identified a band at ~47kD. Reactivity was confirmed in Western blots with mouse thalamus lysates. Mouse Connexin 47-transfected HeLa cells were used in both Immunoprecipitation and Western blot assays. This antibody was also used in immunofluorescence assays using paraformaldehyde-fixed mouse brain. The expected staining pattern is punctate labeling of the oligodendrocytes in the brain.
Applications
- Immunofluorescence (1 - 3 µg/ml)
- Western blot (1 - 3 µg/ml)
- Immunoprecipitation (5 - 10 µg/ml)
Usage
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation. Immunofluorescence: 1-3 ug/ml. Western Blot: 1-3 ug/ml. Immunoprecipitation: 5-10 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee