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Anti-GGA1 Antibody (aa336-352) LS-C19101

Note: This antibody replaces LS-C66860

Ordering

Wt. Vol. Conc. Price
100 µg - - Unavailable

Most Popular GGA1 Antibodies

Anti-GGA1 Antibody (aa336-352) LS-C19101
Rabbit Polyclonal to Human GGA1
Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-GGA1 Antibody (aa1-30) LS-C99679
Rabbit Polyclonal to Human GGA1
Human, Mouse
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-GGA1 Antibody (aa86-135) LS-C151565
Rabbit Polyclonal to Human GGA1
Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Pig
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human GGA1
Human
Western blot, ELISA
Unconjugated

Details

Human GGA1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:500 - 1:3000)
  • ELISA (1:10000 - 1:50000)

Specificity and Use

GGA1 antibody was raised against synthetic peptide from human GGA1.
aa336-352
Amino acids 336-352 of Human GGA-1.
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at 70 kD in size corresponding to GGA1 by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About GGA1

Q9UJY5 NM_013365 NP_037497.1

GGA1 Antibody

GGA1 is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.

Western blot

Western blot
Anti-GGA1 Antibody - Western Blot. Western blot of Affinity Purified anti-GGA1 antibody shows detection of bands at ~100 kD corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells over- expressing the recombinant protein (arrowhead). Approximately 35 ug of lysate was separated on a 4-20% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred overnight at 4? followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: 
Date Requested: 3/29/2017

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