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Anti-GFP Tag Antibody LS-C157772


Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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100% Guaranteed 100% Guaranteed Publications Publications (3)
Mouse Monoclonal (IgG1)
IHC - Paraffin, Immunofluorescence, Western blot


IgG1 Monoclonal
Protein G purified


  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:4000)

Specificity and Use

GFP Tag antibody was raised against purified His-tagged GFP protein.


PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (3)

Localization of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum to the Golgi apparatus. Witola WH, Pessi G, El Bissati K, Reynolds JM, Mamoun CB. The Journal of biological chemistry. 2006 281:21305-11. [PubMed:16704982]
Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases. Yalcin A, Clem BF, Simmons A, Lane A, Nelson K, Clem AL, Brock E, Siow D, Wattenberg B, Telang S, Chesney J. The Journal of biological chemistry. 2009 284:24223-32. [PubMed:19473963] [PMC:PMC2782016]
Glioma-specific cation conductance regulates migration and cell cycle progression. Rooj AK, McNicholas CM, Bartoszewski R, Bebok Z, Benos DJ, Fuller CM. The Journal of biological chemistry. 2012 287:4053-65. [PubMed:22130665] [PMC:PMC3281704]

About GFP Tag


Formalin-fixed and paraffin-embedded mouse skin tissue expression GFP reacted with GFP Tag Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. -ve shows negative staining of the antibody in a wild type mouse, whereas +ve shows positive staining in a mouse expressing GFP under the LGR5 promoter. The staining shows localization of GFP at the bottom of the hair follicle. The antigen was retrieved in citrate pH 6 in water bath for 25 mins.


Immunofluorescence for GFP Tag Antibody. Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing. Secondary goat-anti-mouse and goat-anti-rabbit were conjugated with Alexa546 (Molecular Probes)at 1:200 dilution. Phase contrast enhancement applied. Fluorescent LAMP exposure times as follows: RFP Channel, 0.075 sec; YFP Channel, 1.000 sec; DAPI Channel, 0.020 sec; Phase Channel: 0.300 sec. Microscope: Axiovert 200M inverted microscope (Zeiss) equipped with a PRIOR stage and a NEOFLUAR 100x/1.3 objective lens. Images were collected with a Hamamatsu C4742-95 CCD camera. Chroma Filtersets: TRITC, HQ545/30x - Q570LP - HQ620/60m; DAPI, D350/50x - E420LP - 400DCLP; YFP, Q151LP - HQ500/20x - HQ535/30m. Data courtesy of Seth Robertson, Boston College.

Western blot

Western blot
Western blot of lysate from GFP protein, using GFP Tag Antibody. Antibody was diluted at 1:4000. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.

Requested From: United States
Date Requested: 10/25/2016

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