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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GCKR Antibody LS‑C155498 Best Seller
GCKR antibody LS-C155498 is an unconjugated rabbit polyclonal antibody to GCKR from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C155498-100
100 µl (1 mg/ml)
Unavailable

Popular GCKR Products

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Immunohistochemistry of paraffin-embedded human colon cancer using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, IHC - Paraffin, ELISA
Immunohistochemistry of Human brain using GCKR Polyclonal Antibody at dilution of 1:50.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

GCKR antibody LS-C155498 is an unconjugated rabbit polyclonal antibody to GCKR from human and mouse. Validated for IHC and WB.
About GCKR
Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity. Q14397 NM_001486 NP_001477.2

GCKR Antibody, GKRP Antibody, FGQTL5 Antibody, Glucokinase regulator Antibody, Glucokinase regulatory protein Antibody


Specifications

Target
Human GCKR
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GCKR antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 214 of GCKR (SwissProt Q14397). Percent identity by BLAST analysis: Human (100%); Rat (92%).
Specificity
Human, mouse GCKR
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Normal Colon, using GCKR antibody at 1:500 dilution.
IHC of paraffin-embedded Normal Colon, using GCKR antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GCKR antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GCKR antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Normal Colon, using GCKR antibody at 1:500 dilution.
IHC of paraffin-embedded Normal Colon, using GCKR antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GCKR antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GCKR antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Normal Colon, using GCKR antibody at 1:500 dilution.
IHC of paraffin-embedded Normal Colon, using GCKR antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GCKR antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GCKR antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Normal Colon, using GCKR antibody at 1:500 dilution.
IHC of paraffin-embedded Normal Colon, using GCKR antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GCKR antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GCKR antibody diluted at 1:1000


Requested From: 
Date Requested: 6/17/2018

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