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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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GATA2 Antibody (clone 2D11) LS‑C197276
GATA2 antibody LS-C197276 is an unconjugated mouse monoclonal antibody to human GATA2. Validated for ELISA, IF, RNAi and WB.
Catalog
Size
Price
LS-C197276-100
100 µg
Unavailable

Popular GATA2 Products

GATA2 antibody LS-C31147 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Western blot of GATA2 (arrow) using rabbit polyclonal GATA2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GATA2 gene.
Species: Human
Applications: Western blot, Flow Cytometry
Western blot of GATA2 (arrow) using rabbit polyclonal GATA2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA2 gene (Lane 2).
Species: Human
Applications: Western blot, Flow Cytometry
Antibody
Species: Human
Applications: ICC, Dot Blot
WB of GATA2 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human GATA2, 38.3KD. (162aa tag+ M1-G200). Lane 1: Recombinant Human GATA2 Protein 10ng. Lane 2: Recombinant Human GATA2 Protein 5ng. Lane 3: Recombinant Human GATA2 Protein 2.5ng.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
Applications: Western blot

Product Description

GATA2 antibody LS-C197276 is an unconjugated mouse monoclonal antibody to human GATA2. Validated for ELISA, IF, RNAi and WB.
About GATA2
GATA2 is a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants. P23769 NM_032638 NP_116027.2

GATA2 Antibody, DCML Antibody, GATA binding protein 2 Antibody, GATA-binding protein 2 Antibody, MONOMAC Antibody, NFE1B Antibody


Specifications

Target
Human GATA2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [2D11]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
  • RNA interference
Immunogen
GATA2 antibody was raised against gATA2 (AAH18988, aa1-102 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human GATA2
Usage
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

GATA2 monoclonal antibody (M01), clone 2D11 Western Blot analysis of GATA2 expression in HeLa NE.
GATA2 monoclonal antibody (M01), clone 2D11 Western Blot analysis of GATA2 expression in HeLa NE.

Western blot

Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged GATA2 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GATA2 is approximately 0.03 ng/ml as a capture antibody.

RNA interference

Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunofluorescence

Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

GATA2 monoclonal antibody (M01), clone 2D11 Western Blot analysis of GATA2 expression in HeLa NE.
GATA2 monoclonal antibody (M01), clone 2D11 Western Blot analysis of GATA2 expression in HeLa NE.

Western blot

Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged GATA2 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GATA2 is approximately 0.03 ng/ml as a capture antibody.

RNA interference

Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunofluorescence

Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

GATA2 monoclonal antibody (M01), clone 2D11 Western Blot analysis of GATA2 expression in HeLa NE.
GATA2 monoclonal antibody (M01), clone 2D11 Western Blot analysis of GATA2 expression in HeLa NE.

Western blot

Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged GATA2 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GATA2 is approximately 0.03 ng/ml as a capture antibody.

RNA interference

Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunofluorescence

Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

GATA2 monoclonal antibody (M01), clone 2D11 Western Blot analysis of GATA2 expression in HeLa NE.
GATA2 monoclonal antibody (M01), clone 2D11 Western Blot analysis of GATA2 expression in HeLa NE.

Western blot

Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged GATA2 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GATA2 is approximately 0.03 ng/ml as a capture antibody.

RNA interference

Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.


Requested From: 
Date Requested: 6/24/2018

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