Rat GABRG2

Rabbit

Rat, Human, Mouse, Bovine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

A 30 AA peptide sequence near the N-Terminus of rat GAG2 subunit (2). The peptide was coupled to KLH. Location of epitope is extracellular.

N-Terminus

Recognizes GABA receptor A, Gamma 2. The 30 AA rat control peptide is 100% conserved in bovine, human and mouse GAG2. It is ~80% conserved in chicken GAB2. Significant homology is also seen with rat GAG1 (64%), ratGAG3 (55%). It is not clear if anti-GAG21 actually reacts with other gamma subunits. No significant homology is observed with other GAA subunits (alpha, beta etc.). Antibody cross-reactivity of anti-GAG21 in various species has also not been studied.

• Western blot (1:1000 - 1:5000)
• ELISA (1 µg/ml)

Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure antibody using ECL. ELISA. Control peptide can be used to coat ELISA plates at 1 ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1 ug/ml for affinity pure).

Neat serum, 0.05% sodium azide, 40% glycerol.

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P18507 NCBI: NM_000816 NP_000807.2