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Anti-G3BP1 / G3BP Antibody (clone 2F3) IHC-plus™ LS-B4491

Note: This antibody replaces LS-C105095

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular G3BP1 / G3BP Antibodies

Anti-G3BP1 / G3BP Antibody (clone 2F3) IHC-plus™ LS-B4491
Mouse Monoclonal [clone 2F3] (IgG1,k) to Human G3BP1 / G3BP
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA, RNA interference
Unconjugated
Immunohistochemistry Image
Anti-G3BP1 / G3BP Antibody (aa136-185) IHC-plus™ LS-B5637
Rabbit Polyclonal (IgG) to Human G3BP1 / G3BP
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2F3] (IgG1,k) to Human G3BP1 / G3BP
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA, RNA interference
Unconjugated

Details

Human G3BP1 / G3BP
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2F3]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
  • RNA interference

Specificity and Use

G3BP1 / G3BP antibody was raised against g3BP (AAH06997, 214 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control A-431. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. RNAi Knockdown (Antibody validated).

Packaging

PBS, pH 7.2. Sourced in Ascites.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About G3BP1 / G3BP

Q13283 NM_005754 NP_005745.1

G3BP1 Antibody, HDH-VIII Antibody, G3BP-1 Antibody, G3BP Antibody, GAP binding protein Antibody, HDH VIII Antibody

May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency.

Immunohistochemistry

Immunohistochemistry
Anti-G3BP1 / G3BP antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4491 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-G3BP1 / G3BP antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4491 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-G3BP1 / G3BP antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4491 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to G3BP on HeLa cell (antibody concentration 10 ug/ml).

Western blot

Western blot
G3BP monoclonal antibody clone 2F3 Western blot of G3BP expression in A-431.

Western blot

Western blot
Western blot of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody clone 2F3.

RNA interference

RNA interference
Western blot of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody clone 2F3. GAPDH ( 36.1 kD ) used as specificity and.
 

Requested From: United States
Date Requested: 12/2/2016

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