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Anti-FST / Follistatin Antibody IHC-plus™ LS-B7434

Note: This antibody replaces LS-C104466, LS-C42595

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FST / Follistatin Antibodies

Anti-FST / Follistatin Antibody (Biotin) LS-C104465
Rabbit Polyclonal to Human FST / Follistatin
Human
Western blot, ELISA
Biotin Conjugated
Anti-FST / Follistatin Antibody IHC-plus™ LS-B7434
Rabbit Polyclonal (IgG) to Human FST / Follistatin
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human FST / Follistatin
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated

Details

Human FST / Follistatin
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (8 - 12 µg/ml)

Specificity and Use

FST / Follistatin antibody was raised against e.coli derived recombinant Human Follistatin.
Human Follistatin
Immunohistochemistry: LS-B7434 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100 ng/ml), a concentration of 8-12 ug/ml of this antibody is required. ELISA: To detect Human Follistatin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Follistatin (LS-C104465) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Follistatin. Western Blot: To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Follistatin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About FST / Follistatin

P19883 NM_006350 NP_006341.1

FST Antibody, Activin-binding protein Antibody, Follistatin isoform FST317 Antibody, Follistatin Antibody, Fs Antibody

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin.

Immunohistochemistry

Immunohistochemistry
Anti-FST / Follistatin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7434 concentration 5 ug/ml.

Western blot

Western blot
Western Blot (non-reducing) of Follistatin antibody LS-B7434

Western blot

Western blot
Western Blot (reducing) of Follistatin antibody LS-B7434

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of Follistatin antibody LS-B7434

Requested From: United States
Date Requested: 12/7/2016

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