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Anti-FOXI1 Antibody (aa221-270) IHC-plus™ LS-B950

Note: This antibody replaces LS-C30135
Catalog Size Price
LS-B950-50 50 µg Unavailable

Related Products

39 FOXI1 Antibodies

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Anti-FOXI1 Antibody LS-C15274
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Anti-FOXI1 Antibody (clone 2C1) LS-C175141
Mouse Monoclonal [clone 2C1] (IgG1) to Human FOXI1
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Immunohistochemistry Image
Anti-FOXI1 Antibody (clone 5D3) LS-C175144
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Anti-FOXI1 Antibody (clone 1D4) LS-C175146
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Immunohistochemistry Image
Anti-FOXI1 Antibody (aa111-350, clone 2B8) LS-C336930
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100% Guaranteed
Rabbit Polyclonal to Human FOXI1
Human
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human FOXI1
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

FOXI1 antibody was raised against synthetic peptide located between aa221-270 of human FOXI1 (Q12951, NP_036320). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Marmoset, Tamarin (92%).
LS-E11295 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B950. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa221-270
Human FOXI1
Immunohistochemistry: LS-B950 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B950 was determined to be 2.5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About FOXI1

Q12951 NM_012188 NP_036320.2

FOXI1 Antibody, Forkhead-related activator 6 Antibody, FREAC6 Antibody, Fork-head homolog-3 Antibody, Forkhead-like 10 Antibody, FREAC-6 Antibody, HNF-3/fork-head homolog 3 Antibody, HFH-3 Antibody, FKHL10 Antibody, Forkhead box protein I1 Antibody, HFH3 Antibody, FKH10 Antibody, Forkhead box I1 Antibody, HNF-3/fork-head homolog-3 Antibody

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene.

Immunohistochemistry

Immunohistochemistry
Anti-FOXI1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B950 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-FOXI1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B950 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
FOXI1 antibody LS-B950 Western blot of Fetal kidney lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal Kidney Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 8/18/2017

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