Human FOXH1

Sheep

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

E. coli-derived recombinant human rhFoxH1 (aa 164 - 272).

aa164-272

Recognizes human FoxH1. Species sequence homology: mouse 55%. less than 1% cross-reactivity with rhFoxB2.

• ICC (10 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Western blot, Immunocytochemistry and Direct ELISA. Western blot: 0.1-0.2 ug/ml. The detection limit is ~5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect human FoxH1 in human BG0IV stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at room temperature for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml. The detection limit is ~0.2 ng/well. Hybridoma: Produced in sheep immunized with purified, E. coli-derived, recombinant human FoxH1. (rhFoxH1; aa 164-272; Accession # O75593).

Lyophilized PBS, 5% Trehalose

Phosphate Buffered Saline (PBS)

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O75593 NCBI: NM_003923 NP_003914.1