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Anti-FOXA1 Antibody (aa429-439) IHC-plus™ LS-B3596

Note: This antibody replaces LS-C54847

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FOXA1 Antibodies

Anti-FOXA1 Antibody (aa395-444) IHC-plus™ LS-B946
Rabbit Polyclonal to Human FOXA1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Pig, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-FOXA1 Antibody (clone 1B1) IHC-plus™ LS-B6100
Mouse Monoclonal [clone 1B1] (IgG2a,k) to Human FOXA1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-FOXA1 Antibody (clone 3C1) IHC-plus™ LS-B6101
Mouse Monoclonal [clone 3C1] (IgG1,k) to Human FOXA1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human FOXA1
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Pig, Xenopus, Zebrafish
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human FOXA1
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Pig, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:8000) & (0.3 - 1 µg/ml)
  • ELISA (1:8000)

Specificity and Use

FOXA1 antibody was raised against synthetic peptide C-GVYSRPVLNTS from the C-terminus of human FOXA1 (NP_004487.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Pig, Opossum, Xenopus, Zebrafish (100%); Lizard (91%); Pufferfish (82%).
LS-E25558 - Lyophilized - 100 µg - $145.00
aa429-439
Human FOXA1.
Immunohistochemistry: LS-B3596 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3596 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About FOXA1

P55317 NM_004496 NP_004487.2

FOXA1 Antibody, Forkhead box A1 Antibody, Forkhead box protein A1 Antibody, HNF-3-alpha Antibody, HNF-3A Antibody, TCF-3A Antibody, TCF3A Antibody, HNF3A Antibody, Transcription factor 3A Antibody

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity).

Immunohistochemistry

Immunohistochemistry
Anti-FOXA1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3596 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-FOXA1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3596 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.3 ul/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/6/2016

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