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Anti-Fibroblasts Antibody LS-C66110

Catalog Size Price
LS-C66110-100 100 µg (0.1 mg/ml) Unavailable

Related Products

8 Fibroblasts Antibodies

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100% Guaranteed
Mouse Monoclonal (IgG1) to Human Fibroblasts
Human
Immunofluorescence, Flow Cytometry (applications tested for the base form of this product only)
Unconjugated

Details

Human Fibroblasts
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Ion exchange chromatography
Unmodified

Applications

  • Immunofluorescence (1:100 - 1:200)
  • Flow Cytometry (1:100)
  • (applications tested for the base form of this product only)

Specificity and Use

Fibroblasts antibody was raised against whole human thymic stoma cells.
Recognizes human fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue (e.g. fibrosarcomas) {Haynes et al, 1984}. Useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.
Suitable for use in Immunofluorescence, FACS, and the assessment of thymic epithelial culture contamination with fibroblasts and the identification of cells of mesodermal origin in the fetus. Immunofluorescence: 1:100-1:200 (acetone fixed tissue). FACS: 1:100 (fresh or acetone fixed tissue; do not use trypsin to prepare adherent cell lines, use a non-enzymatic detachment procedure.

Packaging

PBS, 10 mM sodium azide, 1 mg/ml BSA.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Fibroblasts


Requested From: 
Date Requested: 8/23/2017

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