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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FGFR2 / FGF Receptor 2 Antibody (aa22‑51) LS‑C97519
FGF Receptor 2 antibody LS-C97519 is an unconjugated rabbit polyclonal antibody to FGF Receptor 2 (FGFR2) from human and mouse. Validated for Flow, ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C97519-400
400 µl
Unavailable

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Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

FGF Receptor 2 antibody LS-C97519 is an unconjugated rabbit polyclonal antibody to FGF Receptor 2 (FGFR2) from human and mouse. Validated for Flow, ICC, IF, IHC and WB.
About FGFR2 / FGF Receptor 2
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. P21802 NM_000141 NP_000132.3

FGFR2 Antibody, BEK Antibody, BBDS Antibody, Bacteria-expressed kinase Antibody, CD332 antigen Antibody, CFD1 Antibody, Craniofacial dysostosis 1 Antibody, CEK3 Antibody, Fgf bek Antibody, FGF receptor Antibody, Hydroxyaryl-protein kinase Antibody, Jackson-Weiss syndrome Antibody, JWS Antibody, FGF Receptor 2 Antibody, KSAM Antibody, KGFR Antibody, Soluble FGFR4 variant 4 Antibody, BFR-1 Antibody, TK14 Antibody, TK25 Antibody, CD332 Antibody, ECT1 Antibody, K-SAM Antibody


Specifications

Target
Human FGFR2 / FGF Receptor 2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • ICC (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Blocking Peptide
LS-E9138 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C97519. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa22-51
Specificity
This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human FGFR2.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).
C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).

Immunofluorescence

Confocal immunofluorescence of FGFR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of FGFR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of lysates from HeLa, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysates from HeLa, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

FGFR2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).
FGFR2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).

Western blot

FGFR2 Antibody western blot of HeLa cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).
FGFR2 Antibody western blot of HeLa cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).

Flow Cytometry

FGFR2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FGFR2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).
C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).

Immunofluorescence

Confocal immunofluorescence of FGFR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of FGFR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of lysates from HeLa, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysates from HeLa, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

FGFR2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).
FGFR2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).

Western blot

FGFR2 Antibody western blot of HeLa cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).
FGFR2 Antibody western blot of HeLa cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).

Flow Cytometry

FGFR2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FGFR2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).
C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).

Immunofluorescence

Confocal immunofluorescence of FGFR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of FGFR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of lysates from HeLa, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysates from HeLa, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

FGFR2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).
FGFR2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).

Western blot

FGFR2 Antibody western blot of HeLa cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).
FGFR2 Antibody western blot of HeLa cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).

Flow Cytometry

FGFR2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FGFR2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).
C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).

Immunofluorescence

Confocal immunofluorescence of FGFR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of FGFR2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of lysates from HeLa, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of lysates from HeLa, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody R22. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

FGFR2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).
FGFR2 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).

Western blot

FGFR2 Antibody western blot of HeLa cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).
FGFR2 Antibody western blot of HeLa cell line lysates (35 ug/lane). The FGFR2 antibody detected the FGFR2 protein (arrow).

Flow Cytometry

FGFR2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FGFR2 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/23/2018

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