Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone M19B2] (IgG1) to Human FGFR1 / FGF Receptor 1
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Mouse Monoclonal [clone M2F12] (IgG2a) to Human FGFR1 / FGF Receptor 1
Human, Mouse, Rat
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Human FGFR1 / FGF Receptor 1
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [M2F12]
Protein G purified
IHC - Paraffin (10 µg/ml)
IHC - Frozen
Western blot (1 µg/ml)
Specificity and Use
Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and C-terminal glu-phe sequences.
Reacts with the NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a. Reacts with human, rat, and mouse receptor. Other species untested.
Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculo-viral, or native FGFr1. Immunoprecipitation: native, nuclear, recombinant, and ligand-labeled FGFr1. Immunohistochemistry: frozen sections of human glioblastomas, paraformaldehyde fixed cells and nuclei, human myocardial biopsies, FFPE human tissues.
PBS, pH 7.4
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB.