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Anti-FceR1 / Fc Epsilon Receptor 1 Antibody (clone 9E1) IHC-plus™ LS-B3150

Note: This antibody replaces LS-C57917, LS-C57921, LS-C57918

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular FceR1 / Fc Epsilon Receptor 1 Antibodies

Anti-FceR1 / Fc Epsilon Receptor 1 Antibody (clone 9E1, RPE) LS-C57919
Mouse Monoclonal [clone 9E1] (IgG2b) to Human FceR1 / Fc Epsilon Receptor 1
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
RPE Conjugated
Immunohistochemistry Image
Anti-FceR1 / Fc Epsilon Receptor 1 Antibody (clone 9E1) IHC-plus™ LS-B3150
Mouse Monoclonal [clone 9E1] (IgG2b) to Human FceR1 / Fc Epsilon Receptor 1
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 9E1] (IgG2b) to Human FceR1 / Fc Epsilon Receptor 1
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human FceR1 / Fc Epsilon Receptor 1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [9E1]
Unconjugated. Also available conjugated with RPE.
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:25 - 1:50)
  • ELISA
  • (applications tested for the base form of this product only)

Specificity and Use

Recognizes the human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two disulfide linked gamma subunits. Clone Recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain. Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.
Immunohistochemistry: LS-B3150 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3150 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About FceR1 / Fc Epsilon Receptor 1


Immunohistochemistry

Immunohistochemistry
Anti-FceR1 / Fc Epsilon Receptor 1 antibody IHC of human mast cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3150 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry
Staining of human peripheral blood cells with MOUSE ANTI HUMAN FC EPSILON R1, showing staining of eosinophils. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/9/2016

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