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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
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Mouse Monoclonal (IgG1,k) to Bovine FBN1 / Fibrillin 1
Bovine
IHC, Immunofluorescence, Western blot
Biotin Conjugated
Details
Bovine FBN1 / Fibrillin 1
Mouse
Bovine (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Biotin
Purified
Unmodified
Applications
IHC (1:50 - 1:100)
Immunofluorescence
Western blot (1 µg/ml)
Specificity and Use
Microfibrils from zonular apparatus of bovine eye.
Recognizes bovine fibrillin (Mr 350kD). Species cross-reactivity: Human.
Immunohistochemistry. Immunofluorescence. Western Blot. Electron microscopic immunolocalization. Immunohistochemistry: 1:50-1:100 (30-60 min at RT). Western Blot: 1 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Packaging
PBS, sodium azide.
Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Regulates osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively.