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Anti-FAP Alpha Antibody (Internal) LS-C154234

Ordering

Wt. Vol. Conc. Price
100 µg - 1.1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular FAP Alpha Antibodies

Anti-FAP Alpha Antibody (Extracellular Domain) IHC-plus™ LS-A8027
Rabbit Polyclonal to Human FAP Alpha
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse FAP Alpha
Mouse
Western blot, ELISA
Unconjugated

Details

Mouse FAP Alpha
Rabbit
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:500 - 1:2000)
  • ELISA

Specificity and Use

FAP Alpha antibody was raised against this affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.
Internal
This antibody is known to react with mouse FAP protein. A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88% homology with the immunizing sequence. Reactivity with FAP from other sources has not been determined.
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 88 kD in size corresponding to FAP by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About FAP Alpha

Q12884 NM_004460 NP_004451.2

FAP Antibody, DPPIV Antibody, FAP alpha Antibody, Fibroblast activation protein Antibody, FAPA Antibody, Seprase Antibody

Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth.

Western blot

Western blot
Anti-FAP Antibody - Western Blot. Western blot of affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kD, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1000. Personal communication, S. Kim, NCI, Bethesda, MD.
 

Requested From: United States
Date Requested: 12/2/2016

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