Human, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
FAM116A antibody was raised against synthetic peptide located between aa398-447 of human DENND6A (Q8IWF6, NP_689891). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Platypus (100%); Galago, Marmoset, Mouse, Turkey, Zebra finch, Chicken, Seabass (92%); Stickleback (91%); Lizard (85%); Xenopus (84%).
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.