Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
F9 Antibody, Christmas Disease Antibody, Coagulation factor IX Antibody, Factor IX Deficiency Antibody, FIX Antibody, HEMB Antibody, F9 p22 Antibody, Factor 9 Antibody, Factor IX Antibody, Factor IX F9 Antibody, FIX F9 Antibody, Haemophilia B Antibody, THPH8 Antibody, Christmas factor Antibody, Hemophilia B Antibody, PTC Antibody, Serine protease Antibody
F9 / Factor IX is a vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII.
Western Blot analysis of F9 expression in transfected 293T cell line by F9 monoclonal antibody (M01), clone 2C9.Lane 1: F9 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of F9 transfected lysate using anti-F9 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F9 rabbit polyclonal antibody.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged F9 is approximately 0.1 ng/ml as a capture antibody.
Western blot analysis of F9 over-expressed 293 cell line, cotransfected with F9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with F9 monoclonal antibody (M01), clone 2C9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.