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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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F2 / Prothrombin / Thrombin Antibody IHC‑plus™ LS‑B2559
Note: This antibody replaces LS-C38625
Thrombin antibody LS-B2559 is an unconjugated mouse monoclonal antibody to human Thrombin (F2 / Prothrombin). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2559-50
50 µg (1 mg/ml)
Unavailable

Popular F2 / Prothrombin / Thrombin Products

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Applications: Western blot, ELISA
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Applications: Western blot, ELISA, Inhibition

Product Description

Thrombin antibody LS-B2559 is an unconjugated mouse monoclonal antibody to human Thrombin (F2 / Prothrombin). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About F2 / Prothrombin / Thrombin
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Finally, peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. P00734 NM_000506 NP_000497.1

F2 Antibody, Prothrombin B-chain Antibody, Coagulation factor II Antibody, FACTOR II Antibody, Prothrombin Antibody, PT Antibody, RPRGL2 Antibody, Serine protease Antibody, THPH1 Antibody, Thrombin Antibody


Specifications

Target
Human F2 / Prothrombin / Thrombin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (10 µg/ml)
  • ELISA (5 - 10 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human Prothrombin.
Specificity
Recognizes human Prothrombin, prethrombin-1, fragment 2, meizothrombin. Inhibits clotting and prothrombin activation.
Usage
Immunohistochemistry: LS-B2559 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2559 was determined to be 5 ug/ml.
Presentation
Supplied as a liquid in 50% glycerol, ddH2O. Sourced from ascites.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2559 concentration 5 ug/ml.
Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2559 concentration 5 ug/ml.

Immunohistochemistry

Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2559 concentration 5 ug/ml.
Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2559 concentration 5 ug/ml.

Immunohistochemistry

Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2559 concentration 5 ug/ml.
Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2559 concentration 5 ug/ml.

Immunohistochemistry

Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2559 concentration 5 ug/ml.
Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2559 concentration 5 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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