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Anti-F2 / Prothrombin / Thrombin Antibody IHC-plus™ LS-B2559

Note: This antibody replaces LS-C38625


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)

F2 / Prothrombin / Thrombin Antibody LS-B2559 Images

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Anti-F2 / Thrombin antibody IHC of human liver.

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Mouse Monoclonal (IgG2a) to Human F2 / Prothrombin / Thrombin
IHC - Paraffin, Western blot, ELISA


Human F2 / Prothrombin / Thrombin
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Ion exchange chromatography


IHC - Paraffin (5 µg/ml), Western blot (10 µg/ml), ELISA (5 - 10 µg/ml)

Specificity and Use

F2 / Prothrombin / Thrombin antibody was raised against human Prothrombin.
Recognizes human Prothrombin, prethrombin-1, fragment 2, meizothrombin. Inhibits clotting and prothrombin activation.
Immunohistochemistry: LS-B2559 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2559 was determined to be 5 ug/ml.


Supplied as a liquid in 50% glycerol, ddH2O.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About F2 / Prothrombin / Thrombin

P00734 NM_000506 NP_000497.1

F2 Antibody, Prothrombin B-chain Antibody, Coagulation factor II Antibody, FACTOR II Antibody, Prothrombin Antibody, PT Antibody, RPRGL2 Antibody, Serine protease Antibody, THPH1 Antibody, Thrombin Antibody

Coagulation factor II (Prothrombin) is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia.


Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2559 concentration 5 ug/ml.

Requested From: United States
Date Requested: 4/18/2015

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