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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EYA1 Antibody (N‑Terminus) LS‑C201257
EYA1 antibody LS-C201257 is an unconjugated rabbit polyclonal antibody to EYA1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C201257-50
50 µg (1 mg/ml)
Unavailable

Popular EYA1 Products

EYA1 antibody LS-C107835 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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EYA1 Antibody western blot of mouse bladder tissue lysates (35 ug/lane). The EYA1 antibody detected the EYA1 protein (arrow).
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Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

EYA1 antibody LS-C201257 is an unconjugated rabbit polyclonal antibody to EYA1 from human, mouse, rat and other species. Validated for WB.
About EYA1
Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Q99502 NM_000503 NP_000494.2

EYA1 Antibody, BOP Antibody, BOS1 Antibody, Eyes absent homolog 1 Antibody, BOR Antibody


Specifications

Target
Human EYA1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
EYA1 antibody was raised against synthetic peptide from N-Terminus of human EYA1 (E5RIQ7). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Opossum, Guinea pig (100%); Marmoset, Turkey, Chicken, Lizard (92%); Zebra finch, Xenopus, Seabass, Stickleback, Medaka, Pufferfish, Zebrafish (85%).
Epitope
N-Terminus
Specificity
Human EYA1
Presentation
Lyophilized from PBS, 2% sucrose.
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of Human Thyroid tumor. EYA1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Thyroid tumor. EYA1 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human Thyroid tumor. EYA1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Thyroid tumor. EYA1 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human Thyroid tumor. EYA1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Thyroid tumor. EYA1 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human Thyroid tumor. EYA1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Thyroid tumor. EYA1 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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