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Anti-ESET / SETDB1 Antibody (aa1058-1075) IHC-plus™ LS-B720

Note: This antibody replaces LS-C18831


Wt. Vol. Conc. Price
50 µg - 1.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular ESET / SETDB1 Antibodies

Anti-ESET / SETDB1 Antibody (aa1058-1075) IHC-plus™ LS-B720
Rabbit Polyclonal to Human ESET / SETDB1
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-ESET / SETDB1 Antibody (clone 5H6A12) LS-C169282
Mouse Monoclonal [clone 5H6A12] (IgG1) to Human ESET / SETDB1
Human, Monkey, Mouse
IHC, ICC, Immunofluorescence, Western blot, ELISA
Immunofluorescence Image
Anti-ESET / SETDB1 Antibody (C-Terminus, PE) LS-C282651
Rabbit Polyclonal (IgG) to Human ESET / SETDB1
Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (4)
Rabbit Polyclonal to Human ESET / SETDB1
IHC - Paraffin, Western blot, ELISA


Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:2500)
  • ELISA (1:10000 - 1:50000)

Specificity and Use

ESET / SETDB1 antibody was raised against synthetic peptide from human SETDB1.
Amino acids 1058-1075 of human ESET.
Immunohistochemistry: LS-B720 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B720 was determined to be 5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (4)

Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids. Harte PJ, Wu W, Carrasquillo MM, Matera AG. Cytogenetics and cell genetics. 1999 84:83-6. [PubMed:10343109]
SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. Schultz DC, Ayyanathan K, Negorev D, Maul GG, Rauscher FJ. Genes & development. 2002 16:919-32. [PubMed:11959841] [PMC:PMC152359]
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression. Wang H, An W, Cao R, Xia L, Erdjument-Bromage H, Chatton B, Tempst P, Roeder RG, Zhang Y. Molecular cell. 2003 12:475-87. [PubMed:14536086]
Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Sarraf SA, Stancheva I. Molecular cell. 2004 15:595-605. [PubMed:15327775]


Q15047 NM_012432 NP_036564.3

SETDB1 Antibody, H3-K9-HMTase4 Antibody, ESET Antibody, KMT1E Antibody, H3-K9-HMTase 4 Antibody, KG1T Antibody, SET domain, bifurcated 1 Antibody, SET domain bifurcated 1 Antibody, KIAA0067 Antibody, Lysine N-methyltransferase 1E Antibody

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation.


Anti-SETDB1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B720 concentration 5 ug/ml.

Western blot

Western blot
Anti-ESET Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-ESET antibody to detect human ESET present in a 293 whole cell lysate. ~30 ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kD is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 10/25/2016

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