SPFH1 antibody LS-C80925 is an unconjugated rabbit polyclonal antibody to SPFH1 (ERLIN1) from human, mouse, rat and other species. Validated for WB.
Human, Mouse, Rat, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
ERLIN1 / SPFH1 antibody was raised against synthetic peptide located between aa74-123 of human ERLIN1 (O75477, NP_006450). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Horse, Pig, Guinea pig, Zebrafish (100%); Monkey, Goat, Panda, Bovine, Opossum, Turkey, Zebra finch, Chicken, Platypus, Stickleback (92%); Beetle (91%); Xenopus, Sea squirt (84%).
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.