Target
Human ERBB3 / HER3
Synonyms
ERBB3 | C-erbB-3 | C-erbB3 | ErbB-3 | HER3 | p85-sErbB3 | p45-sErbB3 | MDA-BF-1 | ErbB3-S | LCCS2 | p180-ErbB3
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
A GST-HER3 fusion protein corresponding to aa 1283 to 1323 at the C-Terminus of human HER3.
Specificity
Recognizes human HER3 at 180kD. Species cross-reactivity: mouse and rat. Assay by IEP resulted in a single precipitin arc against anti-rabbit serum.
Applications
- Western blot
- Immunoprecipitation
Usage
Suitable for use in Immunoprecipitation and Western Blot. Immunoprecipitation: 5ul. Use 25 ul of Protein A-agarose beads and 1 ml of lysate (lysate contains approximately 1 mg of total protein). Rotate mixture overnight at 4?C and then wash 3x with lysis buffer. Dissolve the resulting bead complex in 20-30 ul of 3xSDS-PAGE sample buffer and load ~15 ul per lane on an 8% polyacrylamide gel. Positive control: Human prostate cell lines (LNCap) or human breast cell line (MDA-453).
Presentation
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee