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Anti-ERBB2 / HER2 Antibody (Cytoplasmic Domain) IHC-plus™ LS-A9398

Note: This antibody replaces LS-C77372, LS-C65536
Catalog Size Price
LS-A9398-50 50 µg (1 mg/ml) Unavailable
Same day shipping In Stock - Same day shipping

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100% Guaranteed In Stock
Rabbit Polyclonal to Human ERBB2 / HER2
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse
IHC - Paraffin
Unconjugated

Details

Human ERBB2 / HER2
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse (tested or 100% immunogen sequence identity)
Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

IHC - Paraffin (10 µg/ml)

Specificity and Use

ERBB2 / HER2 antibody was raised against synthetic 17 amino acid peptide from cytoplasmic domain of human ERBB2 / HER2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Hamster, Horse (100%); Elephant, Pig (94%); Bat (88%).
LS-E29632 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A9398. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Cytoplasmic Domain
Human ERBB2 / HER2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A9398 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A9398 was determined to be 10 ug/ml.

Packaging

PBS, less than 0.1% sodium azide.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
For research use only.

About ERBB2 / HER2

P04626 NM_004448 NP_004439.2

ERBB2 Antibody, C-erb B2/neu protein Antibody, CD340 Antibody, HER2 Antibody, Herstatin Antibody, MLN 19 Antibody, HER-2 Antibody, Neu Antibody, NGL Antibody, Proto-oncogene c-ErbB-2 Antibody, Proto-oncogene Neu Antibody, CD340 antigen Antibody, MLN19 Antibody, p185erbB2 Antibody, TKR1 Antibody

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs).

Immunohistochemistry

Immunohistochemistry
Anti-ERBB2 / HER2 antibody LS-A9398 IHC of human breast carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: 
Date Requested: 10/20/2017

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