Human EPOR / EPO Receptor

Sheep

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

Partial cDNA of the extracellular domain of human EpoR expressed as a GST fusion protein in E. coli.

Extracellular Domain

Recognizes Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival. Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function. AHP1825 has been shown to block Iodine125 labeled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cell in neutralization studies, see for details. AHP1825 is reported as suitable for use in immunocytochemistry. AHP1825 has been reported for use in functional studies. Removal of Sodium Azide is recommended prior to use in functional assays. The recommended concentration for functional assays is 500 ug/ml.

• Western blot (10 µg/ml)
• Immunoprecipitation (10 µg/ml)
• ELISA

PBS, 0.09% Sodium Azide

Store at 4°C. Do not freeze. Store undiluted. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P19235 NCBI: NM_000121 NP_000112.1