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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EPHX4 / Epoxide Hydrolase 4 Antibody (aa71‑120) IHC‑plus™ LS‑B5866
Note: This antibody replaces LS-C111673
Epoxide Hydrolase 4 antibody LS-B5866 is an unconjugated rabbit polyclonal antibody to Epoxide Hydrolase 4 (EPHX4) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5866-50
50 µg
Unavailable

Popular EPHX4 / Epoxide Hydrolase 4 Products

Western blot of extracts from HT-29 cells, using ABHD7 Antibody. The lane on the right is treated with the synthesized peptide.
Species: Human, Mouse
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of EPHX4 / Epoxide Hydrolase 4 antibody.
Species: Mouse
Applications: IHC, Western blot
Human Lung, Respiratory Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western blot of EPHX4 / Epoxide Hydrolase 4 antibody.
Species: Mouse
Applications: IHC, Western blot

Product Description

Epoxide Hydrolase 4 antibody LS-B5866 is an unconjugated rabbit polyclonal antibody to Epoxide Hydrolase 4 (EPHX4) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human EPHX4 / Epoxide Hydrolase 4
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Bat, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa71-120 of human EPHX4 (Q8IUS5, NP_775838). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (100%); Bat, Stickleback, Zebrafish (92%).
Blocking Peptide
LS-E20122 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5866. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa71-120
Specificity
Human EPHX4
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-EPHX4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5866 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EPHX4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5866 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EPHX4 antibody LS-B5866 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EPHX4 antibody LS-B5866 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-EPHX4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5866 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EPHX4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5866 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EPHX4 antibody LS-B5866 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EPHX4 antibody LS-B5866 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-EPHX4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5866 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EPHX4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5866 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EPHX4 antibody LS-B5866 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EPHX4 antibody LS-B5866 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-EPHX4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5866 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EPHX4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5866 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EPHX4 antibody LS-B5866 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EPHX4 antibody LS-B5866 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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