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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EPHB3 / EPH Receptor B3 Antibody (clone 1B3) LS‑C133175
EPH Receptor B3 antibody LS-C133175 is an unconjugated mouse monoclonal antibody to human EPH Receptor B3 (EPHB3). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C133175-100
100 µg (0.5 mg/ml)
Unavailable

Popular EPHB3 / EPH Receptor B3 Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-EPHB3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4091 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

EPH Receptor B3 antibody LS-C133175 is an unconjugated mouse monoclonal antibody to human EPH Receptor B3 (EPHB3). Validated for ELISA, IHC and WB.
About EPHB3 / EPH Receptor B3
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. P54753 NM_004443 NP_004434.2

EPHB3 Antibody, Cek10 Antibody, ETK2 Antibody, HEK2 protein kinase Antibody, Embryonic kinase 2 Antibody, EPH receptor B3 Antibody, EPH-like kinase 2 Antibody, Ephrin type-B receptor 3 Antibody, MDK5 Antibody, Sek4 Antibody, TYRO6 Antibody, EK2 Antibody, EPH-like tyrosine kinase 2 Antibody, EPH-like tyrosine kinase-2 Antibody, HEK2 Antibody, Human embryo kinase 2 Antibody, Tyrosine-protein kinase TYRO6 Antibody


Specifications

Target
Human EPHB3 / EPH Receptor B3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [1B3]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
EPHB3 (NP_004434, 899 a.a. ~ 998 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human EPHB3
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Western blot

Western blot of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.
Western blot of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.

Western blot

EPHB3 monoclonal antibody (M01), clone 1B3 Western blot of EPHB3 expression in HeLa.
EPHB3 monoclonal antibody (M01), clone 1B3 Western blot of EPHB3 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EPHB3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EPHB3 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Western blot

Western blot of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.
Western blot of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.

Western blot

EPHB3 monoclonal antibody (M01), clone 1B3 Western blot of EPHB3 expression in HeLa.
EPHB3 monoclonal antibody (M01), clone 1B3 Western blot of EPHB3 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EPHB3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EPHB3 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Western blot

Western blot of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.
Western blot of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.

Western blot

EPHB3 monoclonal antibody (M01), clone 1B3 Western blot of EPHB3 expression in HeLa.
EPHB3 monoclonal antibody (M01), clone 1B3 Western blot of EPHB3 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EPHB3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EPHB3 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Western blot

Western blot of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.
Western blot of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.

Western blot

EPHB3 monoclonal antibody (M01), clone 1B3 Western blot of EPHB3 expression in HeLa.
EPHB3 monoclonal antibody (M01), clone 1B3 Western blot of EPHB3 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EPHB3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EPHB3 is approximately 0.1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/19/2018

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