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Anti-Eomesodermin / EOMES Antibody LS-C149936

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Eomesodermin / EOMES Antibodies

Anti-Eomesodermin / EOMES Antibody (N-Terminus) IHC-plus™ LS-B1533
Rabbit Polyclonal (IgG) to Human Eomesodermin / EOMES
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Eomesodermin / EOMES Antibody IHC-plus™ LS-B6801
Chicken Polyclonal to Human Eomesodermin / EOMES
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Eomesodermin / EOMES Antibody LS-C149936
Sheep Polyclonal (IgG) to Human Eomesodermin / EOMES
Human
ICC, Western blot, Flow Cytometry
Unconjugated
Immunocytochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human Eomesodermin / EOMES
Human
ICC, Western blot, Flow Cytometry
Unconjugated

Details

Human Eomesodermin / EOMES
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Flow Cytometry

Specificity and Use

Eomesodermin / EOMES antibody was raised against e. coli-derived recombinant human EOMES Gly471-Pro686 Accession # O95936.
Detects human EOMES in Western blots.
Western Blot, 1 µg/ml. Immunocytochemistry, 5-15 µg/ml, Intracellular Staining by Flow Cytometry, 2.5 µg/106 cells.

Packaging

Lyophilized from 0.2 µm filtered PBS, pH 7.4, 5% trehalose
Reconstitute at 0.2 mg/mL in sterile PBS
Store at -20 to -70 C. Stable for 12 months from date of receipt.
For research use only.

About Eomesodermin / EOMES

O95936 NM_005442 NP_005433.2

EOMES Antibody, Eomesodermin Antibody, Eomesodermin homolog Antibody, T box, brain, 2 Antibody, T-box brain protein 2 Antibody, T-box brain2 Antibody, T-brain-2 Antibody, TBR-2 Antibody, TBR2 Antibody

Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

Immunocytochemistry

Immunocytochemistry
EOMES in mesoderm lineage cells differentiated from BG01 V. EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.

Western blot

Western blot
Detection of Human EOMES by Western Blot. Western blot shows lysates of BG01 V human embryonic stem cells untreated(-) or mesoendoderm differentiated (+). PVDF Membrane was probed with 1 ug/ml of Human EOM ES Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for EOMES at approximately 85-105 kD (as indicated). This experiment was conducted under reducing conditions.

Flow Cytometry

Flow Cytometry
Detection of EOMES in Differentiated BG01 V Human Cells by Flow Cytometry. BG01 V human embryonic stem cells differentiated to mesendoderm were stained with Sheep Anti-Human EOM ES Antigen Affinity-purified Polyclonal Antibody or isotype control antibody, followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
 

Requested From: United States
Date Requested: 2/28/2017

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