Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
EOMES in mesoderm lineage cells differentiated from BG01 V. EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Detection of Human EOMES by Western Blot. Western blot shows lysates of BG01 V human embryonic stem cells untreated(-) or mesoendoderm differentiated (+). PVDF Membrane was probed with 1 ug/ml of Human EOM ES Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for EOMES at approximately 85-105 kD (as indicated). This experiment was conducted under reducing conditions.
Detection of EOMES in Differentiated BG01 V Human Cells by Flow Cytometry. BG01 V human embryonic stem cells differentiated to mesendoderm were stained with Sheep Anti-Human EOM ES Antigen Affinity-purified Polyclonal Antibody or isotype control antibody, followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.