Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse (tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
Western blot (1:5000 - 1:20000)
Specificity and Use
ENTPD5 / CD39L4 antibody was raised against recombinant fragment corresponding to a region within amino acids 173 and 428 of ENTPD5 (SwissProt O75356). Percent identity by BLAST analysis: Human (100%); Rat (90%).
Human, mouse ENTPD5
Suggested starting dilutions are as follows: Western blot: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis.