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Anti-EMI2 / FBXO43 Antibody (N-Terminus) IHC-plus™ LS-B369

Note: This antibody replaces LS-C19125

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

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Anti-EMI2 / FBXO43 Antibody (N-Terminus) IHC-plus™ LS-B369
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Unconjugated
Immunohistochemistry Image
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human EMI2 / FBXO43
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human EMI2 / FBXO43
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • ELISA (1:10000 - 1:50000)

Specificity and Use

EMI2 / FBXO43 antibody was raised against synthetic peptide from human FBXO43 / EMI2.
N-Terminus
A region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein.
Immunohistochemistry: LS-B369 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B369 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About EMI2 / FBXO43

Q4G163 Q4G163

FBXO43 Antibody, Early mitotic inhibitor 2 Antibody, EMI2 Antibody, Endogenous meiotic inhibitor 2 Antibody, F-box protein 43 Antibody, ERP1 Antibody, F-box only protein 43 Antibody, FBX43 Antibody

Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (Probable).

Immunohistochemistry

Immunohistochemistry
Anti-FBXO43 / EMI2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B369 concentration 5 ug/ml.

Western blot

Western blot
Anti-Fbp5B Antibody - Western Blot. Western blot of affinity purified anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide. This pre-incubation greatly diminishes specific band recognition. Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells. When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown). Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute.

Requested From: 
Date Requested: 3/25/2017

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