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Anti-ELN / Elastin Antibody IHC-plus™ LS-B2115

Note: This antibody replaces LS-C23007

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ELN / Elastin Antibodies

Anti-ELN / Elastin Antibody IHC-plus™ LS-B2115
Rabbit Polyclonal to Human ELN / Elastin
Human
IHC - Paraffin, Immunofluorescence, ELISA, Radioimmunoassay
Unconjugated
Immunohistochemistry Image
Anti-ELN / Elastin Antibody (clone 10B8) IHC-plus™ LS-B2146
Mouse Monoclonal [clone 10B8] (IgG) to Chicken ELN / Elastin
Chicken, Human, Mouse, Bovine
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ELN / Elastin Antibody LS-C124712
Rabbit Polyclonal (IgG) to Bovine ELN / Elastin
Bovine
IHC, ICC, Western blot, Immunoprecipitation, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ELN / Elastin
Human
IHC - Paraffin, Immunofluorescence, ELISA, Radioimmunoassay
Unconjugated

Details

Human ELN / Elastin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence (1:80)
  • ELISA
  • Radioimmunoassay

Specificity and Use

ELN / Elastin antibody was raised against elastin extracted and purified from human skin
Recognizes human Elastin. Crossreactivity: < 0.1% with human Collagen I, II, III, IV, V, human Fibronectin, or rat Elastin by radioimmunoassay.
Immunohistochemistry: LS-B2115 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2115 was determined to be 2.5 ug/ml. Immunofluorescence: 1:80. Frozen human skin and liver tissues. Positive control: Human skin or liver.

Packaging

PBS buffer, pH 7.2 with no preservatives added
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About ELN / Elastin

P15502 NM_000501 NP_000492.2

ELN Antibody, SVAS Antibody, WBS Antibody, Elastin Antibody, Tropoelastin Antibody, WS Antibody

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.

Immunohistochemistry

Immunohistochemistry
Anti-Elastin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2115 concentration 2.5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Elastin antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2115 concentration 2.5 ug/ml.

Requested From: United States
Date Requested: 12/6/2016

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