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Anti-ELF5 Antibody (aa101-150) IHC-plus™ LS-B5633

Note: This antibody replaces LS-C116040
Catalog Size Price
LS-B5633-50 50 µg (1 mg/ml) Unavailable

Related Products

25 ELF5 Antibodies

Most Popular ELF5 Antibodies

Anti-ELF5 Antibody (aa101-150) IHC-plus™ LS-B5633
Rabbit Polyclonal to Mouse ELF5
Mouse, Rat, Bat, Pig
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-ELF5 antibody IHC of mouse salivary glands. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5633 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ELF5 Antibody (aa191-240) IHC-plus™ LS-B11375
Rabbit Polyclonal (IgG) to Human ELF5
Human, Mouse
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-ELF5 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11375 dilution 1:50.
Anti-ELF5 Antibody LS-C346265
Rabbit Polyclonal (IgG) to Human ELF5
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence analysis of A549 cells.
Anti-ELF5 Antibody (C-Terminus) LS-C352155
Rabbit Polyclonal to Human ELF5
Human, Mouse, Rat, Bovine
ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunofluorescent analysis of ELF5 staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Anti-ELF5 Antibody LS-C412772
Rabbit Polyclonal to Human ELF5
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated

100% Guaranteed
Rabbit Polyclonal to Mouse ELF5
Mouse, Rat, Bat, Pig
IHC, IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Mouse ELF5
Rabbit
Mouse, Rat, Bat, Pig (tested or 100% immunogen sequence identity)
Human, Monkey, Horse, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ELF5 antibody was raised against synthetic peptide located between aa101-150 of mouse Elf5 (Q8VDK3, NP_034255). Percent identity by BLAST analysis: Galago, Mouse, Rat, Elephant, Bat, Pig (100%); Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rabbit, Horse (92%); Orangutan, Tamarin (90%); Dog, Bovine (85%); Guinea pig (84%).
LS-E13288 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5633. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa101-150
Mouse ELF5
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About ELF5

Q9UKW6 NM_001422 NP_001413.1

ELF5 Antibody, ESE2 Antibody, E74-like factor 5 Antibody, ESE-2 Antibody

ELF5 is a member of an epithelium-specific subclass of the Ets transcription factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus.

Immunohistochemistry

Anti-ELF5 antibody IHC of mouse salivary glands. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5633 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ELF5 antibody IHC of mouse salivary glands. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5633 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ELF5 antibody LS-B5633 Western blot of Mouse Kidney lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ELF5 antibody LS-B5633 Western blot of Mouse Kidney lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/23/2018

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