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Anti-ELAVL4 / HuD Antibody (aa35-84) LS-C110595

Catalog	Size	Price
LS-C110595-50	50 µg	Unavailable

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Anti-ELAVL4 / HuD Antibody (Biotin) LS-C525635

Antibody:	Mouse Monoclonal (IgG1,k) to Human ELAVL4 / HuD
Reactivity:	Human, Rat
Application:	IHC, Western blot, ELISA
Format:	Biotin Conjugated

Antibody:	Rabbit Polyclonal to Human ELAVL4 / HuD
Reactivity:	Human, Monkey, Zebrafish
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human ELAVL4 / HuD
Host:	Rabbit
Reactivity:	Human, Monkey, Zebrafish (tested or 100% immunogen sequence identity)
Predicted:	Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

ELAVL4 / HuD antibody was raised against synthetic peptide located between aa35-84 of human ELAVL4 (P26378, NP_068771). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Opossum (100%); Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (92%); Lizard (85%).

Blocking Peptide:

LS-E15030 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110595. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa35-84

Specificity:

Human ELAVL4 / HuD

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About ELAVL4 / HuD

Uniprot: P26378 NCBI: NM_021952 NP_068771.2

Synonyms: ELAVL4 Antibody, ELAV-like protein 4 Antibody, Hu antigen D Antibody, HUD Antibody, PNEM Antibody, Hu-antigen D Antibody

Description: May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR By similarity. Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA.

(More About ELAVL4 / HuD)

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Western blot

ELAVL4 / HuD antibody LS-C110595 Western blot of NCI-H226 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 8/18/2017

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